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Derivation Method for Determining Sorbitol in Fruit Trees  [PDF]
Regina Célia Faria Sim？o Canesin, William Deodato Isique, Salatiér Buzetti, Juliana Aparecida de Souza
American Journal of Plant Sciences (AJPS) , 2014, DOI: 10.4236/ajps.2014.523361
Abstract:
The goal of this study is to describe a methodology for the analysis of D-sorbitol using benzyl alcohol as derivatizing agent and liquid chromatography equipped with a diode-array detector (HPLC-DAD). The study was performed at the University of São Paulo State (UNESP), Ilha Solteira Campus, Brazil. The axillary buds of fruit trees were collected from the UNESP Teaching and Research Farm. Benzyl alcohol was used as derivatizing agent to extract D-sorbitol from the buds. Next, the D-sorbitol underwent solid phase extraction (SPE) using C18 cartridges and was then analyzed by liquid chromatography (HPLC-DAD). The HPLC-DAD derivatization method proposed showed excellent chromatography resolution and high accuracy in the separation of D-sorbitol (derivatized) for the axillary buds of avocado, black mulberry, Japanese pear and peach and also enabled eliminating the use of hazardous derivatizing agents.
Lucratividade do milho em raz？o das fontes, doses e épocas de aplica？？o de nitrogênio
Souza, Juliana Aparecida de;Buzetti, Salatiér;Tarsitano, Maria Aparecida Anselmo;Valderrama, Márcio;
Revista Ceres , 2012, DOI: 10.1590/S0034-737X2012000300005
Abstract: nitrogen is used in larger quantities than any other mineral nutrient in corn. it is also the most limiting to plant growth and development and the nutrient that most increases the production costs. the objective of this study was to evaluate the economic viability using different sources, rates and application times of nitrogen in the production of irrigated corn in selvíria-ms, brazil. the experiment was conducted in an experimental field belonging to unesp - ilha solteira campus, in an acrustox. the treatments consisted of three n sources (entec？, ammonium sulfate and urea), five n rates (0, 50, 100, 150 and 200 kg ha-1) and two application times (applied at sowing or topdressing). the total operating cost (toc), gross revenue (gr), operating profit (op), profitability index (pi), break-even yield, and break-even price were estimated. the results showed that ammonium sulfate and urea were the n sources that allowed satisfactory economic results. rates of 50 kg ha-1 of ammonium sulfate or urea provided higher levels of profitability. the highest levels of profitability were obtained when nitrogen application was carried out at topdressing.
Aduba？？o nitrogenada na cultura do milho safrinha irrigado em plantio direto
Souza, Juliana Aparecida;Buzetti, Salatiér;Teixeira Filho, Marcelo Carvalho Minhoto;Andreotti, Marcelo;Sá, Marco Eustáquio de;Arf, Orivaldo;
Bragantia , 2011, DOI: 10.1590/S0006-87052011000200028
Abstract: the second corn crop is an important economical alternative for agriculture. as this crop removes great amounts of n, it is necessary an appropriate nitrogen fertilization management for obtaining high yields. the objective of this work was to evaluate the effect of sources, doses and times of nitrogen application on agronomic characteristics and productivity of corn grains in an irrigated second crop. the experiment was accomplished in selvíria, mato grosso do sul state, in a red dystrophic latosol irrigated by sprinkle and conducted under no till, in 2007 and 2008. the experiment was arranged in randomized complete blocks design, with 4 repetitions, disposed in a factorial scheme 5x3x2, being: five doses of n (0, 50, 100, 150 and 200 kg ha-1), three sources of n (ammonium sulfonitrate with nitrification inhibitor, ammonium sulfate and urea) applied at sowing or totally at sidedressing in the stage of 6 leaves. the n sources provided similar grain yield. the nitrogen application at sowing was viable, as well as the traditional application of n at sidedressing. the increment of n doses increased the leaf n content, the number of grains per line and per ear and consequently the grain yield. the n doses increased the grain yield of the second crop up to 150 kg ha-1 of n, independently of application time or n sources.
Biological control of Anopheles darlingi, Aedes aegypti and Culex quinquefasciatus larvae using shrimps
Juliana de Carvalho Apolinário Coêlho,Katia Denise Saraiva Bresciani,Willian Marinho Dourado Coelho,Wilma Aparecida Starke Buzetti
- , 2017, DOI: 10.1016/j.parepi.2017.05.002
Abstract: Mosquitoes can act as vectors of important diseases such as malaria, dengue, Zika virus, yellow fever, Chikungunya and Mayaro fever, in addition to filariasis. The use of insecticides, larvicides, bed nets and repellents, besides the use of drugs as chemoprevention and the treatment of the sick are currently the pillars of the control of these vectors. We studied the biological control against of Anopheles darlingi, Aedes aegypti and Culex quinquefasciatus larvae using shrimps of the species M. pantanalense, M. amazonicum, M. brasiliense and M. jelskii. Larvae of mosquitoes were collected from the breeding environment and placed in a 500 and 1000 l tank containing 60 shrimps/m2. The predatory activity was evaluated for 30 days and, in all groups it was observed that 100% of the larvae were consumed in few minutes. In the environment, these same species of crustaceans were released in water bodies with the presence of larvae of these insects. In just 72 h there was a marked reduction of the larvae in the release sites of shrimps. Similarly, there was a reduction in the number of adult mosquitoes caught near the breeding sites, allowing to infer that, in places where the crustaceans were released, the predatory activity on the larvae of mosquitoes was sufficient to reduce the number of adult mosquitoes p ≤ 0,05. This is the first description of the predatory activity of M. pantanalense, M. amazonicum, M. brasiliense and M. jelskii on An. darlingi, A. aegypti and C. quinquefasciatus larvae, constituting an important tool of biological control of these parasites-vectors
Estudo comparativo dos métodos diagnósticos para Leishmaniose Visceral em c？es oriundos de Ilha Solteira, SP
Assis, Juliana de;Queiroz, Nina Marí Gual Pimenta de;Silveira, Rita de Cássia Viveiros da;Nunes, Cáris Maroni;Oliveira, Trícia Maria Ferreira de Sousa;Noronha Junior, Antonio Carlos Faconti de;Neves, Maria Francisca;Machado, Rosangela Zacarias;Buzetti, Wilma Aparecida Starke;
Revista Brasileira de Parasitologia Veterinária , 2010, DOI: 10.4322/rbpv.01901004
Abstract: the purpose of the present work was a comparative study of diagnostic methods for canine visceral leishmaniasis (cvl) using serological methods, enzyme-linked immunosorbent assay (elisa) and indirect fluorescent antibody test (ifat), histochemical (he) and immunohistochemical (imhc) tests using spleen, lymph node and liver canine tissues. in addition, polymerase chain reaction (pcr) was done in blood and in tissues in order to compare and confirm no conclusive and negative diagnosis by the methods above. for this study, 34 dogs were divided according to clinical signs in asymptomatic, oligosymptomatic and polisymptomatic leishmania-infected dogs euthanized by zoonotic disease control center (ccz) from ilha solteira, sp, brazil. the positivism indexes of elisa, imhc, ifat and he were 65.0, 62.0, 56.0 and 56.0%, respectively with the highest numbers of positive dogs in polisymptomatic (92.0%) followed by oligosymptomatic (57.0%) and asymptomatic dogs (12.5%). furthermore, pcr confirmed the positive results and detected dna in tissues from 100% of negative dogs and 89.0% suspects raising the animal positivism index up to 97.0%. in conclusion, pcr was the most sensitive and a valuable method for a definitive cvl diagnosis.
Exames parasitológicos, imunoistoquímicos e histopatológicos para detec？？o de Leishmania chagasi em tecidos esplênicos de c？es com leishmaniose visceral
Tasca, Karen Ingrid;Buzetti, Wilma Aparecida Starke;Tenorio, Michely da Silva;Paulan, Silvana de Cássia;Lima, Flávia Luna;Queiroz, Nina Mari Gual Pimenta de;Machado, Rosangela Zacarias;Oliveira, Tricia Maria Ferreira de Souza;Neves, Maria Francisca;Noronha Jr., Antonio Carlos Faconti de;Assis, Juliana de;
Revista Brasileira de Parasitologia Veterinária , 2009, DOI: 10.4322/rbpv.01801005
Abstract: the purpose of this work was a canine visceral leishmaniasis - cvl study by parasitological direct examination of leishmania (l.) chagasi (imprinting and histological), immunohistochemical test and histopathological analysis using spleen tissues from 34 dogs euthanized by the zoonotic disease control centre from ilha solteira, sp, brazil. according to the clinical signs, the dogs were divided in three groups: asymptomatics (8 dogs), oligosymptomatics (17 dogs) and symptomatics (9 dogs). after the accomplishment of all diagnostic tests, 22 dogs were considered positives (64.7%) and 12 (35.3%) were negatives to cvl. from these positive dogs, 1/22 (4.5%) was asymptomatic, 12/22 (54.5%) were oligosymptomatics and 8/22 (40.1%) were symptomatics. the histopathological study in spleen tissues from positive, especially symptomatic dogs, showed a diffuse chronic inflammation with thickness of capsular and trabecular regions and there was extense morphologic alteration of the red and white pulp by the presence of abundant macrophages full with amastigotes, the granulomatous inflammatory reaction and haemorragic areas. the data of this work from histopathologic examination and direct microcopic visualization of l. (l.) chagasi showed that the spleen was an useful organ to collect sample tissues for cvl diagnosis. the immunostaining detected the highest number of positive dogs and were considered an important and conclusive method to be used in addition to parasitological methods for cvl, particularly in asymptomatic or oligosymptomatic dogs.
VIOLêNCIA E INDISCIPLNA EM MEIO ESCOLAR: ASPECTOS TEóRICO-METODOLóGICOS DA PRODU O ACADêMICA NO PERíODO DE 2000 A 2005
Juliana Aparecida Matias Zechi
Nuances : Estudos sobre Educa？？o , 2011,
Abstract: VIOLêNCIA E INDISCIPLNA EM MEIO ESCOLAR: ASPECTOS TEóRICO-METODOLóGICOS DA PRODU O ACADêMICA NO PERíODO DE 2000 A 2005
Fontes e épocas de aplica？？o de nitrogênio em trigo em sistema plantio direto no cerrado
Silva, Simone Aparecida da;Arf, Orivaldo;Buzetti, Salatiér;Silva, Matheus Gustavo da;
Revista Brasileira de Ciência do Solo , 2008, DOI: 10.1590/S0100-06832008000700015
Abstract: nitrogen is an important nutrient, due to its soil dynamics and crop demand. the amount of n supplied by the soil is however frequently insufficient, and n fertilization is required to obtain maximum yields. this study was conducted with the objective of evaluating different sources and periods of n application in no till wheat. a randomized block design was used in a factorial scheme 2x6 with four replications. the treatments consisteded of the combination of two n sources: urea and entec and six application times: control (without n); n at sowing (s); n 15 days after emergence (dae); n 30 dae; 1/3 of n at s + 2/3 15 dae; and 1/3 of n at s + 2/3 30 dae. the experiment was carried out at an experimental station of the unesp in the county of selvíria - mato grosso do sul state, on a soil previously occupied by cerrado vegetation. the n applied 30 days after plant emergence induced highest grain yields, although statistically there was no difference to the application of 1/3 of n at sowing + 2/3 of n 30 days after plant emergence.
Histochemical and molecular evaluation of the prevalence of Leishmania spp. in hematophagous insects
Katia Denise Saraiva Bresciani,Willian Marinho Dourado Coelho,Wilma Aparecida Starke Buzetti
- , 2016, DOI: 10.1016/j.parepi.2016.04.004
Abstract: The prevalence study of Leishmania spp. in hematophagous insects captured from the environment in bat roosts and pigeon nests, or feeding their hosts (cattle, pigs, horses, dogs and humans) in urban, peri-urban and rural areas, between 2012 and 2014. For this study, the amastigotes present in these insects were detected by histochemical and PCR techniques. Positive gene amplification for Leishmania was found in two horseflies of the species Tabanus importunus collected in the environment, and amastigote forms of Leishmania spp., as well as erythrocytes and leukocytes, were histochemically detected in one of that insect. The other analyzed insects were not positive by PCR our by direct parasitological examination. Only horseflies captured in urban and peri-urban areas were positive. During the collection, no phlebotomine sand flies were captured in rural areas far from the city limits. It can be concluded that the discovery of horseflies positive for Leishmania spp. in urban and peri-urban areas indicates the likelihood that urban areas and their surroundings provide vector parasites with an environment suitable for the spread and consequent perpetuation of the biological cycle of this protozoan
Efeitos de um programa de hidroterapia na press？o arterial e freqüência cardíaca de mulheres idosas sedentárias
Candeloro, Juliana Monteiro;Caromano, Fátima Aparecida;
Fisioterapia e Pesquisa , 2008, DOI: 10.1590/S1809-29502008000100005
Abstract: this study aimed at analysing cardiocirculatory effects (on blood pressure, bp, and heart rate, hr) of a hydrotherapy program of which the purpose was to increase muscle force and flexibility of 16 sedentary, healthy, 65-to-70 year-old women. the program consisted of 32 1-hour sessions of strength and flexibility training exercises, with increasing degree of difficulty in seven phases. the first four sessions were used for adaptation to the aquatic environment and the respective data were not considered. bp and hr were monitored at the end of each phase in four different moments: at rest before diving, at rest 3 minutes after immersion, still immerged at the end of exercises, and at rest, 3 minutes after exiting the pool. collected measures were statistically analysed, and significance level set at p<0.05. results show significant mean at-rest systolic bp decrease of 5.6 mmhg, and mean at-rest diastolic bp decrease of 9.7 mmhg, from the 5th (base) session to the 32nd (last) session; participants' bp classification turned from threshold-normal to normal, according to the brazilian hypertension society. also found was a non-significant 1.0-bpm increase in mean hr. findings suggest that, though hr has not been affected, aquatic strength and flexibility exercises with increasing difficulty do not overload - and may positively affect - cardiocirculatory system in healthy women aged 65 to 70 years old.
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